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| Lee,Ki-Won; Choi,Gi Jun; Kim,Ki-Yong; Yoon,Sei Hyung; Ji,Hee Chung; Park,Hyung Soo; Lim,Young Chul; Lee,Sang-Hoon. |
| In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium; Forage crop; Italian ryegrass; Transgenic plants. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008 |
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| Sandrin,Carla Zuliani; Domingos,Marisa; Figueiredo-Ribeiro,Rita de Cássia Leone. |
| In temperate grasses, fructans are the major storage polysaccharides, being accumulated mainly in mature leaf sheaths, and also in the roots. The partitioning of carbohydrates within different organs regulates plant growth and development. The aim of the present work was to analyze the partitioning of water soluble carbohydrates in five different parts (elongating leaf blades, expanded leaf blades, upper and lower segments of the stubble, and roots) of plants of L. multiflorum cv. Lema, in order to contribute to an understanding of soluble carbohydrates distribution in these plants. Soluble carbohydrates and total fructose were analyzed in plants cultivated during 4 months in a glasshouse, by colorimetric, TLC and HPAEC-PAD techniques. Results showed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fructan; Grass; Italian ryegrass; Sugars. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200006 |
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| Souza,Edicarlos Damacena de; Costa,Sérgio Ely Valadão Gigante de Andrade; Anghinoni,Ibanor; Carvalho,Paulo César de Faccio; Oliveira,Eric Victor Ferreira de; Martins,Amanda Posselt; Cao,Eduardo; Andrighetti,Marcelo. |
| Grazing intensities can influence soil aggregation, which can be temporarily and permanently affected. The objective of this study was to evaluate the aggregate stability in water at the end of a soybean cycle and during pasture development in a crop-livestock integration system under no-tillage and grazing intensities. The experiment was initiated in 2001, in a dystrophic Red Latosol, after soybean harvest. Treatments consisted of pasture (black oat + Italian ryegrass) at heights of 10, 20 and 40 cm, grazed by young cattle, and a control (no grazing), followed by soybean cultivation, in a randomized block design. Soil samples were collected at the end of the soybean cycle (May/2007), during animal grazing (September/2007) and at the end of the grazing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Black oat; Italian ryegrass; Mean weighted diameter; Soil structure. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400033 |
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